Nickel LC50 Experiment 2
study plans
nickel chloride
Review resources prior to handling nickel chloride:
qPCR for Nickel exposure on Botryllus schlosseri
Relative standard curves to determine primer effenciencies.
nickel chloride
qPCR
primers
Analysis of gene expression with quantitative Polymerase Chain Reaction (qPCR) will be a component of the in vivo nickel exposure work conducted in autumn 2023. Four experiments were…
Tunicate Hemolymph Extraction for Media Supplementation
epithelial
primaries
paper
Protocol replicated from following paper.
Nickel Impacts on Primary Epithelial Cell Cultures of Botryllus schlosseri
Analysis of pilot study.
nickel chloride
primary cultures
epithelial
Data below was collected from a pilot study conducted in Autumn 2023 where field-collected individual Botryllus schlosseri were exposed to a low and high treatment of nickel chloride and then were dissected for primary buds. The…
Substrate Options for the in vitro Culture of Botryllus schlosseri Epithelial Cells
cool paper
discussion
Exploring of the physiology of the extra cellular matrix (ECM) of the marine invertebrate, ascidian, Botryllus schlosseri as to identify potential substrates for in vitro primary epithelial cell culturing of the same…
Cell Isolation Trial 18: Cultrex Substrates
epithelial
ampullae
zooids
primary buds
primaries
substrate
In this small scale experiment, resorbing zooids and take over zooids, two distinct asexual developmental stages sourced from the same Botryllus individual…
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