All handling of stock solution and treatments to be done in a chemical fume hood. Once spiked water has been distributed, add animals, parafilm jars, place in secondary container, and move to environmental chamber.
Required Volume of Artificial Seawater
Calculations for spiking artificial seawater. 225 mL of seawater required to mostly submerge glass slide in wide mouth 16 oz Kerr mason jar.
Considerations:
3 replicates
225 mL per jar
6 conditions
Account for 10% loss of water during transferring
Additional 15 mL required for analytical chemistry
Need 4,419 mL of artificial seawater total for exposure, particularly, 759 mL of artificial seawater per condition.
Calculations
Stock solution of NiCl2 (10 g/L) made 04/08/24.
NiCl2 (5g)
Suspended in MilliQ water (500 mL)
0.1 mg/L
\[ C_1*V_1 = C_2*V_2\]\[ 10 g/L * V_1 = 0.0001g/L*0.759L\]\[V_1 = 0.00000759 L = 7.59 uL\] Spike 759 mL of aerated, artificial seawater (16 C) with 7.59 uL of stock NiCl2 solution. Change in salinity negligible (+/- 1 PPT).