Tunicate Hemolymph Extraction for Media Supplementation

Protocol replicated from following paper.

Citations

Raftos DA, Cooper EL, Habicht GS, Beck G. Invertebrate cytokines: tunicate cell proliferation stimulated by an interleukin 1-like molecule. Proc Natl Acad Sci USA. 1991 Nov;88(21):9518–22.

Objective

Background

Organisms

::: {#fig-oranisms layout-ncol=“2”}

Ciona savignyi

Botryllus schlosseri

Protocol