Citation
Hesp K, Van Der Heijden JME, Munroe S, Sipkema D, Martens DE, Wijffels RH, et al. First continuous marine sponge cell line established. Sci Rep. 2023 Apr 8;13(1):5766.
Table 1 Compositions of M1 and OpM1 Media
We would target the OpM1 media that helped produce the first continious sponge cell line.
Questions:
Do marine invertebrates like tunicates produce all the amino acids? Would supplementing with amino acids they already produce make them unwell?
What is the current concentration of salt in my media? What are the ratios of salts to amino acids in the media formulation currently in use (TCM)?
| Ingredient | [x] stock | [x] M1* | [x] OpM1* | Unit | Cat. no | |
|---|---|---|---|---|---|---|
| M199 powder | – | 9.1 | 9.1 | g/L | M3769 | |
| Inorganic salts | NaCl | 275 | 15.4203 | 15.4203 | g/L | 488,662 |
| Tris-HCl | 50 | 3.7688 | 3.7688 | g/L | T5941 | |
| Tris-base | 50 | 2.7838 | 2.7838 | g/L | T1503 | |
| MgCl2 | 200 | 10.0400 | 10.0400 | g/L | M2393 | |
| Na2SO4 | 50 | 0.8170 | 0.8170 | g/L | 238,597 | |
| CaCl2 | 50 | 0.4000 | 0.4000 | g/L | C7902 | |
| KCl | 50 | 0.3021 | 0.3021 | g/L | P5405 | |
| Amino acids | L-Alanine | 5 | 0.0281 | 0.0281 | g/L | A3534 |
| L-Arginine | 5 | 0.0250 | 0.0250 | g/L | A6969 | |
| L-Aspartic acid | 5 | 0.0469 | 0.0469 | g/L | A4534 | |
| L-Asparagine | 5 | 0.1031 | 0.1031 | g/L | A0884 | |
| L-Cysteine | 5 | 0.0406 | 0.0406 | g/L | C6852 | |
| L-Glutamic acid | 5 | 0.0469 | 0.0469 | g/L | G5638 | |
| L-Glutamine | 5 | 0.0625 | 0.0880 | g/L | G8540 | |
| Glycine | 5 | 0.0031 | 0.0031 | g/L | G8790 | |
| L-Histidine | 5 | 0.1094 | 0.1094 | g/L | H8125 | |
| L-Isoleucine | 5 | 0.0500 | 0.0500 | g/L | I2752 | |
| L-Leucine | 5 | 0.0531 | 0.0531 | g/L | L8912 | |
| L-Lysine | 5 | 0.0188 | 0.0188 | g/L | L5626 | |
| L-Methionine | 5 | 0.0656 | 0.0656 | g/L | M9625 | |
| L-Phenylalanine | 5 | 0.0938 | 0.0938 | g/L | P5482 | |
| L-Proline | 5 | 0.0781 | 0.0781 | g/L | P5607 | |
| L-Serine | 5 | 0.0844 | 0.0844 | g/L | S5511 | |
| L-Threonine | 5 | 0.0250 | 0.0250 | g/L | T8441 | |
| L-Tryptophan | 5 | 0.0719 | 0.0719 | g/L | T0254 | |
| L-Tyrosine | 5 | 0.0469 | 0.0469 | g/L | T1145 | |
| L-Valine | 5 | 0.1031 | 0.1031 | g/L | V6504 | |
| AB/M** | Rifampicin | 30 | 0.0300 | 0.0300 | g/L | R3501 |
| Amphotericin B | 0.2500 | 0.0025 | 0.0025 | g/L | A2411 | |
| Vit1 | Ascorbate · Na | 0.5943 | – | 0.0015 | g/L | A4034 |
| Pyruvate · Na | 0.4400 | – | 0.0011 | g/L | P5280 | |
| TE | Na2SiO3 | 1.4210 | – | 0.0018 | g/L | 307,815 |
| ZnSO4 · 7H2O | 6.46E−02 | – | 0.0001 | g/L | Z0251 | |
| GF1 | Lectin from Phaseolus vulgaris (PHA) | 4 | – | 4.72E−02 | g/L | L1668 |
| EGF Recombinant Human Protein | 1.60E−04 | – | 1.89E−06 | g/L | 10605HNAE50 | |
| Insulin-like Growth Factor-1 Protein, Recombinant Human | 8.00E−03 | – | 9.44E−05 | g/L | GF-138 | |
| PFT α | Pifithrin-α | 20 | – | 1.19E−02 | g/L | P4359 |
| PDGF | PDGF-BB Recombinant Mouse Protein | 1.00E−01 | – | 8.69E−05 | g/L | PMG0044 |
| RPMI | RPMI 1640 Vitamin solution (100x) | – | – | 3.11 | µl/mL OpM1 | R7256 |
| FBS | Fetal Bovine Serum, qualified, One Shot™, US origin | – | – | 4.35 | µl/mL OpM1 | A3160501 |
| ITS-X | Insulin Transferrin Selenium Ethanolamine | – | – | 11.18 | µl/mL OpM1 | 51,500,056 |
| LM-1 | Lipid Mixture-1 (100x) | – | – | 11.18 | µl/mL OpM1 | L0288 |