Get larger sized Botryllus schlosseri (BSc) colonies growing in the lab.
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Botryllus schlosseri Health Monitoring Data Sheet
Week 02 of the Spring 2023 Quarter Summary
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Asexual and sexual developmental stages of Botryllus schlosseri
Figure from Kowarsky et al., 2021
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Wild Colony Intake Protocol
Week 01 of the Spring Quarter Summary
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Snail Primary Cell Cultures
Summary
On 2023-03-27 I began a trial run of primary cultures from freshwater snails (species unknown). The main purpose of this was to receive training on using the inverted microscope and practice a general protocol for primary cell culturing. The observed protocol has been documented in my physical lab notebook found in the UW Tacoma lab. For all cultures regardless of species, dissection will occur in the main lab in a “non-sterile” environment. For Botryllus schlosseri this will occur under the microscope for the microdissection of individual zooids. Tissue will be placed in a 15 mL centrifuge tube containing liquid media (and likely antibiotics) for transfer to the biosaftey cabinet in the building next door (TPS).
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